effect of antioxidant combinations on sperm quality of cross breed rams during liquid storage

Authors

fatemeh soltanpour

gholamali moghaddam

reza asadpour

sayyed abbas rafat

abstract

the aim of this study was to evaluate the effects of the addition of antioxidants (vitamin e, β- mercaptoethanol and their combination) to semen extender.  progressive motility, viability and ph of fresh ram semen were examined. ejaculate samples were collected with artificial vagina from three ghezel-merinose rams (3 years old) during non- breeding reproduction season. semen was diluted with a citrate-base extender containing group1,without antioxidants,  group2,vitamin e 5mm,  group3, β- mercaptoethanol (β-me) 50mm  and group4,  vitamin e(5mm) &amp;m β-mercaptoethanol 50m. diluted semen was cooled to 5º c and storage at same temperature for 72h. semen was investigated after 24, 48 and 72h. result showed that progressive motility and viability in control group were higher than other treatment groups. the progressive motility in vitamin e supplemented group was significantly higher than other groups supplemented with antioxidant. the addition of vitamin e to the extender increased (p< 0.05) sperm viability compared to other groups supplemented with antioxidants.  progressive motility and viability were significantly higher than other (p< 0.05) in the vitamin e group. viability and progressive motility were significantly higher in vitamin e &amp; β-me groups than in β-me. in conclusion vitamin e supplementation of citrate –egg yolk extender improved progressive motility and viability fresh sperm during storage at 5º c for 72h.  sperm storage time had a significant effect on sperm viability and progressive motility.  based on this study, there were no combinations more efficient at combating semen quality than control group

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Effect of Antioxidant Combinations on Sperm Quality of Cross Breed Rams During Liquid Storage

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

full text

Effect of Antioxidant Combinations on Sperm Quality of Cross Breed Rams During Liquid Storage

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

full text

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

effect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars

این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...

Antioxidant effect of ascorbic acid on the quality of Cobia (Rachycentron canadum) fillets during frozen storage

The effect of aqueous solutions of ascorbic acid (AA) on the rancidity development in Cobia (Rachycentron canadum) fillets during frozen storage was studied. Cobia fillets were treated with ascorbic acid (AA 0.25% and AA 0.5%) then stored at -18°C up to 6 months. Rancidity development was measured by several biochemical indices including free fatty acids (FFA), peroxide value (PV), an...

full text

My Resources

Save resource for easier access later


Journal title:
international journal of advanced biological and biomedical research

Publisher: casrp publishing company

ISSN 2383-2762

volume 2

issue 3 2014

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023